285 Anti-tumor effect of recombinant lentivirus gv493-htert-shrna on malignant melanoma

Telomerase reverse transcriptase as the core component of telomere and most common non-coding region mutation gene in melanoma, may play a significant role development melanoma. The aim this study is to investigate anti-tumor effect human telomerase (hTERT) knockdown malignant melanoma its potential clinical value. We employed hTERT strategy detected expression target by transcriptase-quantitative polymerase chain reaction western blot analysis. Then investigated effects performing cell proliferation, apoptosis, migration invasion assays A375 cells lines vitro observing growth via xenografts mouse model vivo. Knockdown decreased ability invasion, significantly increased apoptosis were arrested G0/G1 phase (P<0.05). In mice, inhibited metastasis It confirmed that essential for tumorigenesis expected be novel or related biomarker treatment